Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 660
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation available

Product Specific Information

The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with cytokines such as TNF alpha to inhibit proliferation of normal and transformed cells.
This antibody is suitable for intracellular staining followed by flow cytometric analysis. Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. Recommended usage ≤0.6 µg per test (100 µL final volume).
Alexa Fluor™ 700 emits at 723 nm and is excited with a red laser (633 nm). Ensure instrument compatibility (typically requires 685 LP mirror and 710/20 filter).
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation and immune interferon produced mainly by T lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits. IFN gamma displays species-specific biological activity; human IFN gamma does not cross-react with mouse.
Functions include antiviral and antiproliferative activity, induction of MHC class I/II, macrophage activation, and enhanced immunoglobulin secretion. It acts through IFN gamma receptor I and II via the JAK-STAT pathway. Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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