Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), PE, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 41 publications

Product Specifications

Additional Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 615, 737, 805
• Brilliant Violet 421, 711
• eFluor 450
• FITC
• NovaFluor Red 685, Red 700, Yellow 700
• PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone, but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported:
The BC96 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested; usable at 5 µL (0.125 µg) per test. A test is defined as the amount of antibody required to stain a cell sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD25 (IL2RA)
CD25 encodes the interleukin-2 receptor alpha chain, a component of the high-affinity IL-2 receptor complex along with IL2RB and IL2RG. This receptor mediates IL-2–dependent proliferation of T and B lymphocytes. IL2RA expression is crucial for immune regulation, and mutations can lead to immune dysregulation such as IL-2 receptor alpha deficiency. IL-2 acts as a growth and differentiation factor and regulates cell death, stimulating B cell proliferation and NK cell activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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