Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), PE, eBioscience
인간 CD25(IL-2Rα) 단백질을 인식하는 BC96 단클론 항체로, PE 형광표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. CD25 발현 세포의 활성화 및 분화 연구에 적합합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 41 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BC96 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1659683 |
Additional Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 615, 737, 805
- Brilliant Violet 421, 711
- eFluor 450
- FITC
- NovaFluor Red 685, Red 700, Yellow 700
- PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
Description:
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone, but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Applications Reported:
The BC96 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested; usable at 5 µL (0.125 µg) per test. A test is defined as the amount of antibody required to stain a cell sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Fluorescence Characteristics:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2RA)
CD25 encodes the interleukin-2 receptor alpha chain, a component of the high-affinity IL-2 receptor complex along with IL2RB and IL2RG. This receptor mediates IL-2–dependent proliferation of T and B lymphocytes. IL2RA expression is crucial for immune regulation, and mutations can lead to immune dysregulation such as IL-2 receptor alpha deficiency. IL-2 acts as a growth and differentiation factor and regulates cell death, stimulating B cell proliferation and NK cell activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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