Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PE, eBioscience
Mouse CD25(IL-2Rα) 인식 단클론 항체로, PE 형광표지되어 Flow Cytometry에 최적화됨. IL-2 수용체의 고/저친화 결합 억제 연구에 유용. 0.125 µg/test 권장, 565/576 nm 형광 특성. 4°C 보관, 동결 금지.
- 카탈로그번호
- 12-0251-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PE, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 201 publications |
| Functional Assay | - | 4 publications |
| Miscellaneous (PubMed) | - | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark. Do not freeze. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465606 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
Description:
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B cells and by activated mature T and B lymphocytes. CD25 alone binds IL-2 with low affinity but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both receptor classes. CD25 is involved in lymphocyte differentiation and activation/proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse splenocytes and Con A-stimulated splenocyte cultures. Recommended ≤0.125 µg/test (in 100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration for best performance.
Excitation / Emission: 488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Target: CD25 (IL2RA, Interleukin-2 receptor alpha chain)
CD25 is part of the IL-2 receptor complex regulating proliferation of T and B lymphocytes. The receptor is a heterotrimeric complex (IL2RA, IL2RB, IL2RG) forming high-affinity IL-2 binding sites. IL2RA homodimers form low-affinity receptors, while IL2RB homodimers form medium-affinity receptors. IL2 expression in mature thymocytes is monoallelic, ensuring precise regulation. IL-2 acts as a growth and differentiation factor and regulates apoptosis. It also stimulates B cell proliferation, enhances NK cell activity, and suppresses granulocyte-macrophage colony formation. IL2RA deficiency is associated with immune dysregulation disorders.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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