Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), PE, eBioscience
CD25-4E3 단클론 항체는 인간 및 비인간 영장류 CD25(IL-2 수용체 α) 검출용으로 설계된 PE 결합 항체입니다. 활성화된 T세포 및 조절 T세포 분석에 적합하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 4°C 암조건 보관, 동결 금지.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), PE, eBioscience
Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD25-4E3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043826 |
Available Formats
- APC
- APC-eFluor 780
- Brilliant Violet 605
- eFluor 450
- FITC
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
This CD25-4E3 monoclonal antibody reacts to human CD25, also known as the low-affinity interleukin (IL)-2 receptor alpha.
CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages.
This receptor subunit associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor.
IL-2 receptor signaling is linked to lymphocyte proliferation and survival.
CD25 serves as a marker for Hodgkin’s disease and is associated with autoimmune diseases such as Type I diabetes.
This monoclonal antibody is cross-reactive to rhesus monkey.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD25 (IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation.
The IL2 receptor complex (IL2RA, IL2RB, IL2RG) forms a high-affinity receptor for IL2.
Homodimeric IL2RA results in low-affinity binding, IL2RB in medium-affinity.
IL2 expression in mature thymocytes is monoallelic, enabling precise gene regulation.
IL2 is produced mainly by mature T cells and functions as a growth and differentiation factor, regulating cell death and immune responses.
Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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