Thermo Fisher Scientific CD25 Monoclonal Antibody (P4A10), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P4A10

Conjugate

PE PE PE

View additional formats

• eFluor 660
• FITC
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670337

Product Specific Information

Description: This P4A10 monoclonal antibody reacts with canine CD25, the 55-kDa interleukin-2 receptor alpha (IL-2Ralpha) chain. As in humans and mice, CD25 in dogs is expressed by early T and B lineage progenitors as well as by activated mature T and B lymphocytes. CD25 associates with the IL-2R beta (CD122) and gamma (CD132) chains to form the high-affinity IL-2 receptor. On its own, CD25 binds IL-2 with low affinity. Along with CD4 and Foxp3, CD25 is a major marker used to define regulatory T cells.

The P4A10 antibody has been reported to recognize the same epitope as the anti-human CD25 monoclonal antibody ACT-1, but with greater affinity.

Applications Reported: This P4A10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P4A10 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.