Thermo Fisher Scientific proNGF Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:300–1:2,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:300–1:2,000

Miscellaneous (PubMed)

• Publication: View 1 publication

Product Specifications

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: proNGF.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. NGF binds to specific cell surface receptors present as both high and low affinity sites. Only the high affinity form is capable of signal transduction.
Cross-linking studies with ¹²⁵I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low affinity receptor forms an Mr 100,000 complex.
Full-length cDNA clones for both human and rat NGF receptor genes have been produced and sequenced, showing >90% homology.
NGF exhibits nerve growth stimulating activity and regulates growth and differentiation of sympathetic and certain sensory neurons. Mutations in the NGF gene are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF gene expression is linked to allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.