Thermo Fisher Scientific NGF Polyclonal Antibody
NGF 단백질을 인식하는 토끼 다클론 항체로, Western blot, IHC, 억제 분석 등에 사용 가능. 인간, 생쥐, 랫트 반응성. 친화 크로마토그래피로 정제된 동결건조 형태. NGF 관련 신경세포 생존 및 분화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | - |
| Immunohistochemistry (IHC) | 1–5 µg/mL | - |
| Inhibition Assays (IA) | 1–5 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Native beta-nerve growth factor (Ngfb, beta-NGF) purified from mouse submandibular gland |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: NGF.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. NGF binds to specific high- and low-affinity receptors on cell surfaces, but only the high-affinity receptor mediates signal transduction. Cross-linking studies with 125I-NGF show that the high-affinity receptor in rat PC12 cells forms a 158 kDa complex with NGF, while the low-affinity receptor forms a 100 kDa complex. Full-length cDNA clones for both human and rat NGF receptor genes have been sequenced, showing over 90% homology. NGF plays a critical role in nerve growth stimulation and regulation of sympathetic and sensory neuron differentiation. Mutations in the NGF gene are linked to hereditary sensory and autonomic neuropathy type 5 (HSAN5), and NGF dysregulation is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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