Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Red 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7 is a 40 kDa receptor belonging to the immunoglobulin gene superfamily. The N-terminal region (aa1–107) is homologous to Ig kappa light chain sequences, while the proline-rich C-terminal region forms a stalk projecting the Ig domain.

CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, natural killer cells, a small subset of normal B cells, and malignant B cells. Cross-linking of CD7 modulates T and NK cell activity, affecting calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 interacts with phosphoinositol 3'-kinase, leading to D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high levels of CD7 can generate lymphoid cells (B, NK, dendritic) but not myeloid or erythroid cells, distinguishing primitive lymphoid progenitors from pluripotent stem cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Normal human peripheral blood cells at ≤5 µL (0.8 µg)/test (100 µL final volume, 10^5–10^8 cells/test). Optimize antibody titration for best results.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), required for optimal staining. Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10^3–10^8 cells).

NovaFluor conjugates utilize Phiton™ technology, offering engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. Its N-terminal region is homologous to Ig kappa light chains, and its proline-rich extracellular domain forms a stalk projecting the Ig domain. CD7 is expressed on most T lymphocytes, NK cells, and subsets of B cells, including malignant forms. It plays a role in modulating immune cell activity and is an important marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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