Thermo Fisher Scientific 6X His Epitope Tag Polyclonal Antibody, HRP
6X His 태그 단백질 검출용 HRP 결합 폴리클로날 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. Rabbit IgG 기반으로 높은 특이성과 재현성 제공. His-tag 단백질의 정량 및 정성 분석에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:5,000 |
| Immunoprecipitation (IP) | 1:2,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from rabbit serum immunized with 6X His epitope tag peptide (H-H-H-H-H-H) conjugated to KLH using maleimide |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles. Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid. Dilute only before immediate use.
- This affinity-purified antibody specifically recognizes the 6X His motif and detects over-expressed His-tagged proteins in various assays.
- In Western blotting of bacterial extracts, it does not cross-react with endogenous proteins.
- Recognizes His-tag (His-His-His-His-His-His) fused to either N- or C-terminus of target proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly attached to the N- or C-terminus of recombinant proteins to enable purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection and purification of recombinant proteins using antibodies specific to the tag sequence. His-tagged proteins can be purified by Ni²⁺-NTA affinity chromatography due to the strong and selective binding of histidine residues to nickel ions.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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