Thermo Fisher Scientific Phosphothreonine Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho Site Specific affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20°C prior to opening.

Aliquot contents and freeze at -20°C or below for extended storage.

Centrifuge product if not completely clear after standing at room temperature.

This product is stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).

Target Information

Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.