Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, HRP

Applications and Tested Dilutions

Product Specifications

Immunogen

This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to amino acids 410–419 of human c-Myc.

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Rabbit Serum.

Target Information

Myc is a proto-oncogene that encodes a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation. The encoded protein forms a heterodimer with the transcription factor MAX, binding the E-box DNA consensus sequence to regulate target gene transcription. Gene amplification is frequently observed in human cancers, and translocations are associated with Burkitt lymphoma and multiple myeloma. Translation may initiate from both an upstream non-AUG (CUG) and a downstream AUG start site, producing two isoforms with distinct N-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.