Thermo Fisher Scientific Phosphotyrosine Polyclonal Antibody, HRP
HRP 결합된 rabbit polyclonal 항체로, phosphotyrosine 인산화 단백질 검출에 적합합니다. Western blot 및 ELISA에 사용 가능하며, 고순도의 affinity chromatography 정제 제품입니다. -20°C 보관 시 안정적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH. |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Stable for several weeks at 4°C as an undiluted liquid (dilute only prior to immediate use).
- For extended storage, aliquot contents and freeze at -20°C or below.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years.
While phosphorylation of specific tyrosine residues is a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression, and oncogenic transformation, its role in differentiation and gap junction communication remains under active research.
Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable for studying tyrosine-phosphorylated proteins and their biochemical pathways.
The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical protocols where secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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