Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 246 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Request custom conjugation

Product Specific Information

Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form Mac-1 (alphaM-beta2 integrin) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. The antibody also cross-reacts with human CD11b, allowing detection on human monocytes, granulocytes, and NK cell subsets.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Use ≤0.25 µg/test (100 µL final volume). Optimal concentration should be empirically determined.

Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended detection filter: 610/20 band pass (PE-Texas Red® equivalent).

• Light Sensitivity: Protect from light (photo-oxidation sensitive).
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
• Excitation: 488–561 nm
• Emission: 607 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11b (Integrin alpha-M, ITGAM):
A 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. Expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia. Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b expression varies by cell type and increases during monocyte maturation. Commonly used as a microglial marker in nervous tissue.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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