Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), PE-eFluor 610, eBioscience
인간 CD14 단백질을 인식하는 61D3 단클론 항체로, PE-eFluor 610 형광체가 결합된 형광유세포분석용 시약입니다. 모노사이트 및 대식세포 표지에 적합하며, 488–561 nm 레이저로 여기됩니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 61-0149-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), PE-eFluor 610, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- View 55 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 61D3 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565/606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574534 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue & Yellow Series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- Request custom conjugation
Product Specific Information
The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
- Recommended Use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Fluorochrome Information:
PE-eFluor® 610 is excitable by 488–561 nm lasers and emits at 607 nm. Recommended detection filter: 610/20 band pass (PE-Texas Red® equivalent).
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using:
Excitation/Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a receptor for lipopolysaccharide (LPS) in complex with LBP, and exists in both membrane-bound and soluble forms. Soluble CD14 (sCD14) plays a role in neutralizing LPS and modulating immune responses, including apoptotic cell binding and inflammatory regulation.
Dysfunction of CD14 is associated with conditions such as Mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.