Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 20 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710 / 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

Description:
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gamma RIII), a low-affinity receptor for IgG (50–80 kDa). CD16A (FcγRIIIA) is expressed on macrophages and NK cells, while CD16B (FcγRIIIB) is found on neutrophils. CD16 binds IgG immune complexes, primarily IgG1 and IgG3, initiating ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested on normal human peripheral blood cells.
Use 5 µL (0.125 µg) per test in 100 µL final volume.
Applicable cell count: 10⁵–10⁸ cells/test.

Fluorophore Information:
PE-eFluor® 610 is excitable with 488–561 nm lasers and emits at 607 nm.
Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
Light sensitive—protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Optical Properties:

• Excitation: 488–561 nm
• Emission: 607 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Gene: CD16 (FCGR3A)
Encodes a receptor recognizing the Fc portion of IgG, mediating immune complex clearance and ADCC.
FCGR3A (CD16A) is membrane-anchored on NK cells, while FCGR3B (CD16B) is PI-linked on neutrophils.
Mutations are linked to immunodeficiency 20, viral infection susceptibility, systemic lupus erythematosus, and neonatal neutropenia.
Alternative splicing produces multiple isoforms.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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