Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-eFluor 610, eBioscience

Applications

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 450/506/660, FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine series, PerCP-Cyanine5.5, Super Bright series, and custom conjugation available.

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except for terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II that associates with the BCR, mediating signaling processes such as tyrosine phosphorylation, calcium flux, and B cell proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Optimize antibody concentration empirically for best performance.

Fluorophore Information:
PE-eFluor® 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended filter: 610/20 bandpass (equivalent to PE-Texas Red®). Ensure instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54) with minimal brightness loss.

Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and certain myeloid leukemia cells. It serves as a key co-receptor for BCR signaling, lowering activation thresholds and regulating B cell proliferation. Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity. It is a critical marker for B cell lineage and leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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