Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), PE-eFluor 610, eBioscience
인간 CD19를 인식하는 SJ25C1 단클론 항체로, PE-eFluor 610 형광체가 결합된 유세포분석용 항체입니다. B세포 표지에 적합하며, 488–561 nm 레이저로 여기됩니다. 광민감성이 있으므로 어둡게 보관하며, 4°C에서 보관 권장.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- Publications: View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SJ25C1 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% Sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815290 |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 605 / 650
- eFluor 450 / 506
- NovaFluor Blue 610-70S / 660-120S / 690
- NovaFluor Red 685 / 725 / 755
- NovaFluor Yellow 590 / 610 / 690 / 755
- PE / PE-Cyanine5.5 / PE-Cyanine7
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. It forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to mediate signaling, phosphorylation, calcium flux, and proliferation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test (empirically determined).
Fluorochrome Information:
- Excitation: 488–561 nm
- Emission: 607 nm
- Recommended filter: 610/20 band pass (PE-Texas Red equivalent)
- Compatible lasers: Blue, Green, Yellow-Green
- Light sensitivity: Protect from light (photo-induced oxidation sensitive)
Fixation:
Samples can be fixed in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-57)
Store up to 3 days at 4°C in the dark with minimal brightness loss.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on nearly all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor with the B cell receptor (BCR), regulating B cell activation and proliferation. Mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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