Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 610-30S, 660-40S
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤1.0 µg/test recommended
• Laser Compatibility: Violet (405 nm) laser required for BV650 detection
• Light Sensitivity: Protect vial and samples from light
• Fixation Compatibility: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution; not compatible with methanol-based fixation

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize non-specific interactions.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It regulates Src-family kinases and is essential for lymphocyte development and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains and cell-type-specific expression. CD45 also modulates integrin-mediated signaling and is involved in apoptosis-related DNA fragmentation.

For Research Use Only. Not for use in diagnostic procedures.