Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450–660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The RA3-6B2 monoclonal antibody recognizes the exon A-restricted isoform of mouse CD45 (220 kDa). CD45R/B220 epitope is primarily expressed on B cell lineage from early Pro-B to mature B cells, and also on some activated T cells, LAK cells, NK progenitors, and T cells in lpr/lpr mice.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL sample volume, 10⁵–10⁸ cells/test).
• Fluorochrome: Brilliant Violet™ 650 (BV650) emits at 649 nm, excited by 405 nm violet laser. Ensure cytometer compatibility.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dyes to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
• Compatibility: Not compatible with methanol-based fixation.
• Excitation/Emission: 407 nm / 649 nm
• Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family involved in cell growth, differentiation, mitotic cycle, and oncogenic transformation. It is expressed in hematopoietic cells and regulates T- and B-cell receptor signaling.
CD45 exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. It plays roles in lymphocyte development, antigen signaling, integrin-mediated signaling, and apoptosis.
CD45RA is an isoform with restricted expression among lymphoid subtypes.
Associated diseases include Severe Combined Immunodeficiency (T-cell negative, B-cell negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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