Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Brilliant Violet 650, eBioscience
CD27 단백질을 인식하는 LG.7F9 클론의 단클론 항체로, Brilliant Violet 650 형광으로 표지됨. 인간, 생쥐, 랫트 반응성. Flow cytometry에 최적화되어 있으며, T세포 및 B세포 상호작용 연구에 적합. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), Brilliant Violet 650, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Species Reactivity
- Human
- Mouse
- Rat
Host / Isotype
- Armenian hamster / IgG
Recommended Isotype Control
Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet™ 650, eBioscience™
Product Specifications
| 항목 | 내용 |
|---|---|
| Class | Monoclonal |
| Type | Antibody |
| Clone | LG.7F9 |
| Conjugate | Brilliant Violet™ 650 |
| Excitation/Emission Max | 407 / 646 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925677 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 786
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and a subpopulation of B cells (mainly memory B cells). In mouse, CD27 is found on nearly all thymocytes except CD46-CD8- precursors. CD27 binds CD70 and plays an important role in T cell–B cell interaction and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤1.0 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimal titration recommended.
Brilliant Violet™ 650 (BV650) emits at 649 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).
BV650 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD27 is a 50 kDa member of the TNF receptor superfamily (including CD40, CD30). It regulates cell proliferation and death. CD27/CD70 expression is confined to lymphocytes, with CD27 highly expressed upon antigen receptor ligation and CD70 requiring co-stimulatory signals. CD27 forms a disulfide-linked homodimer on thymocytes, peripheral T cells, and memory B cells. Activation via TCR-CD3 increases CD27 expression and releases soluble CD27 (sCD27, 28–32 kDa), a prognostic marker for B cell malignancies. CD27-binding protein (SIVA) mediates apoptosis. CD27 dysfunction is associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
For Research Use Only. Not for use in diagnostic procedures.
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