Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The 104 monoclonal antibody recognizes the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤ 1.0 µg/test (100 µL final volume). Cell numbers between 10⁵–10⁸ cells/test are recommended.
• Optimization: Antibody titration is recommended for best performance.
• Fluorochrome Info: Brilliant Violet™ 650 (BV650) emits at 649 nm and is suitable for cytometers with a 405 nm violet laser.
• Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect from light to avoid photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.
• Excitation: 407 nm
• Emission: 649 nm
• Laser: Violet Laser

Trademark Notice: BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It plays a critical role in lymphocyte development and antigen signaling by regulating Src-family kinases.

CD45.2 has multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains. Isoform expression varies by cell type and physiological state. CD45.2 also contributes to integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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