Thermo Fisher Scientific M-CSF Monoclonal Antibody (26730)
인간 M-CSF를 특이적으로 인식하는 Mouse IgG2a 단일클론 항체로 Western blot, ICC/IF, 중화 실험에 적합합니다. E. coli 유래 재조합 단백질로부터 제작되었으며, 교차 반응성이 낮고 고순도 Protein A/G 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Neutralization (Neu) | 0.005–0.020 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 26730 |
| Immunogen | E. coli-derived recombinant human M-CSF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609575 |
Product Specific Information
- In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M-CSF.
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a critical survival factor for monocyte, macrophage, and osteoclast progenitor cell development. It induces VEGF secretion by macrophages, facilitating endothelial progenitor mobilization and neovascularization.
M-CSF exists as multiple bioactive isoforms differing in potency and stability. The full-length protein is membrane-bound and can be cleaved into soluble forms. It is a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms.
Binding of M-CSF to its receptor c-Fms (CSF-1R or CD115) triggers receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations.
Human M-CSF shows activity on mouse cells, whereas mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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