Thermo Fisher Scientific Phosphotyrosine Recombinant Rabbit Monoclonal Antibody (ARC1703)
Phosphotyrosine 인산화 타이로신 잔기를 특이적으로 인식하는 재조합 토끼 단일클론 항체입니다. Western blot, ELISA, Immunoprecipitation 등 다양한 응용에 적합합니다. HEK293 세포에서 발현된 고순도 항체로, 세포 신호전달 및 암 관련 연구에 활용됩니다.
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Thermo Fisher Scientific Phosphotyrosine Recombinant Rabbit Monoclonal Antibody (ARC1703)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4I9N5 |
| Immunogen | A random peptide library phosphorylated peptide around Tyr |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898156 |
Product Specific Information
Positive Samples: Jurkat, NIH/3T3, C6
Target Information
Tyrosine phosphorylation plays a critical role in transducing mitogenic signals from transmembrane receptors and in transformation by oncogene tyrosine kinases. Phosphorylation of specific tyrosine residues is a key mechanism in normal mitogenesis, cell cycle progression, and oncogenic transformation, while its roles in differentiation and gap junction communication remain under active study.
Antibodies that specifically recognize phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and their biochemical pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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