Thermo Fisher Scientific NEFM Recombinant Rabbit Monoclonal Antibody (JM11-20)
NEFM 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템에서 생산된 Protein A 정제 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM11-20 |
| Immunogen | Synthetic peptide within Human NEFM (aa 651–692) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809890 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, especially in large axons.
They consist of three major subunits:
- NF-M (medium)
- NF-H (high)
- NF-L (light)
These subunits share a coiled-coil core domain but differ in N- and C-terminal regions.
NF-M tail-mediated interactions are essential for axonal size and cytoskeletal architecture.
Phosphorylation and O-GlcNAcylation of NF-M regulate its function and filament formation.
Antibodies against neurofilament subunits are valuable neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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