Thermo Fisher Scientific ATP5A1 Recombinant Rabbit Monoclonal Antibody (JM110-04)
ATP5A1 단백질을 표적하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG 형식의 항체로, Protein A 정제 및 안정적 저장 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM110-04 |
| Immunogen | Synthetic peptide within Human ATP5A1 aa 191–234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809886 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
The ATP5A1 gene encodes a subunit of mitochondrial ATP synthase, responsible for ATP synthesis using the proton gradient across the inner membrane during oxidative phosphorylation.
ATP synthase comprises two complexes:
- F1: Soluble catalytic core
- Fo: Membrane-spanning proton channel
The catalytic portion includes five subunits (alpha, beta, gamma, delta, epsilon) with stoichiometry of 3 alpha, 3 beta, and one each of the others.
The proton channel consists of subunits a, b, and c.
This gene encodes the alpha subunit of the catalytic core.
Alternative splicing generates different isoforms, and pseudogenes are located on chromosomes 9, 2, and 16.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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