Thermo Fisher Scientific TIA-1 Recombinant Rabbit Monoclonal Antibody (JM42-11)
Recombinant rabbit monoclonal antibody recognizing TIA-1 protein. Validated for WB, IHC, ICC, IP, and RIP applications. Offers high specificity, reproducibility, and lot-to-lot consistency. Suitable for human and mouse samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| Immunoprecipitation (IP) | 1–2 µg/sample | View 1 publication |
| RNA Immunoprecipitation (RIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM42-11 |
| Immunogen | Synthetic peptide within Human TIA1 aa 353–386 (UniProt ID: P31483-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809892 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Noggin is involved in multiple developmental processes, including neural tube fusion and joint formation. Organ morphogenesis is initiated by epithelial stem cell downgrowth, guided by signals from WNT proteins (e.g., WNT3A) and Noggin, a bone morphogenetic protein inhibitor.
Mutations in Noggin are associated with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1), both characterized by joint fusion defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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