Thermo Fisher Scientific GAD67 Polyclonal Antibody
GAD67 단백질을 인식하는 Thermo Fisher Scientific의 염소 폴리클로날 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, 인간에 반응하고 다른 종에서도 예측 반응성을 보입니다. 고순도 정제 및 안정적 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (PDSPQRREKLHK) corresponding to the internal amino acids of GAD1 (aa 526–538). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10987356 |
Product Specific Information
- Predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
GAD-65 and GAD-67 are members of the group II decarboxylase family responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid.
Both GADs are found in the brain:
- GAD-65 localizes to synaptic vesicle membranes in nerve terminals.
- GAD-67 is distributed throughout the cell and responsible for basal GABA synthesis.
When GABA demand increases, GAD-65 transiently activates to assist in production. Loss of GAD-65 impairs GABA neurotransmission, while loss of GAD-67 is lethal.
Two isoforms exist for GAD-67 due to alternative splicing: GAD-67 (predominant) and GAD-25 (minor).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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