Thermo Fisher Scientific Tramadol Polyclonal Antibody

Applications

• ELISA (ELISA)
  Tested Dilution: 5–10 µg/mL

Product Specifications

Product Specific Information

• Working Concentration: 5 µg/mL
• Sensitivity: 10 ng/mL Tramadol produces 96% inhibition in a competitive ELISA employing Tramadol polyclonal antibody.

Target Information

Tramadol is only found in individuals that have used or taken this drug. It is a narcotic analgesic proposed for moderate to severe pain and may be habituating.
Tramadol and its O-desmethyl metabolite (M1) are selective, weak OP3-receptor agonists. Opiate receptors are coupled with G-protein receptors and regulate synaptic transmission via G-proteins that activate effector proteins such as adenylate cyclase and cAMP at the inner surface of the plasma membrane.
Opioids decrease intracellular cAMP by inhibiting adenylate cyclase, leading to reduced release of nociceptive neurotransmitters such as substance P, GABA, dopamine, acetylcholine, and noradrenaline.
The analgesic properties of Tramadol are attributed to norepinephrine and serotonin reuptake blockade in the CNS, which inhibits pain transmission in the spinal cord.
The (+) enantiomer has higher affinity for the OP3 receptor and preferentially inhibits serotonin uptake and enhances serotonin release, while the (−) enantiomer preferentially inhibits norepinephrine reuptake by stimulating α(2)-adrenergic receptors.
(Source: PubChem Compound Database; CID=33741)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.