Thermo Fisher Scientific Phospho-PKC mu (Ser910) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of serine 910 (R-V-S(p)-I-L) derived from Human PKD/PKC-mu if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC mu,uniProtId:Q15139-1,ncbiNodeId:9606,antigenRange:908-912,antigenLength:912,antigenImageFileName:PA5-37694_PKC_mu_Q15139-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37694_PKC_mu_Q15139-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554302

Product Specific Information

A suggested positive control for Western blot is 293 or HUVEC cells; suggested positive control for ICC/IF is Hela cells.

Target Information

The PKC family of serine/threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. PKC mu activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.