Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), APC, eBioscience
인간 CD7 단백질을 인식하는 단일클론 항체로, APC 형광체로 결합되어 유세포분석(Flow Cytometry)에 최적화됨. T세포, NK세포, 림프구 표지 연구에 활용 가능. 고순도 친화 크로마토그래피 정제 및 안정적 PBS/BSA 버퍼 제공.
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Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), APC, eBioscience™
Applications
Flow Cytometry (Flow): Tested dilution 5 µL (0.125 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio124-1D1 (124-1D1) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10671279 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant Violet 650
- eFluor 450
- FITC
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 700 / 725 / 755
- NovaFluor Yellow 610 / 700
- PE, PE-Cyanine5.5, PE-Cyanine7
- Super Bright 436 / 600 / 702
- Request custom conjugation available
Product Specific Information
The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily.
CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, NK cells, and a subset of B cells. Cross-linking CD7 modulates T cell and NK activity through calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates directly with phosphoinositol 3-kinase, inducing D-3 phosphoinositides and tyrosine phosphorylation.
A clonogenic subpopulation of CD34(+) CD38(-) cord blood cells expressing CD7 shows lymphopoietic capacity, generating B cells, NK cells, and dendritic cells.
CD7 also cooperates with CD28 in Treg function; deficiency in both reduces Treg numbers and suppressive activity.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member expressed on immature and mature T lymphocytes, T cell leukemias, NK cells, and subsets of B cells.
It modulates T and NK cell activity and serves as an important marker in leukemia diagnostics.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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