Thermo Fisher Scientific CD8a Monoclonal Antibody (OX8), APC, eBioscience
Rat CD8α 인식용 OX8 단클론 항체로, APC 형광 표지되어 Flow Cytometry에 적합. 고순도 친화 크로마토그래피 정제, 0.2 mg/mL 농도. 4°C 암조건 보관, 다양한 형광 포맷 제공.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (OX8), APC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 0.06 µg/test
- Publications: View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX8 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1659704 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The OX8 monoclonal antibody reacts with rat CD8 alpha, a member of the immunoglobulin superfamily that heterodimerizes with CD8 beta to form the CD8 antigen. CD8 is expressed on CD4+CD8+ thymocytes, MHC class I-restricted peripheral T cells, and some macrophages.
CD8 binds to MHC class I molecules during antigen presentation and participates in T-cell receptor signal transduction via association with kinase Lck.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of rat splenocytes (≤0.06 µg/test)
- Recommended Use: Carefully titrate for optimal performance
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family involved in T cell activation and signal transduction. CD8 is primarily expressed on T cells as disulfide-linked CD8α/CD8β heterodimers and functions as a co-receptor for MHC class I molecules.
CD8 also exists as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells. Interaction with MHC-I/peptide complexes recruits Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs play a key role in pathogen and tumor clearance. Differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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