Thermo Fisher Scientific CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), Alexa Fluor 488, eBioscience
마우스 CD252(OX40 Ligand)을 인식하는 RM134L 단클론 항체로, Alexa Fluor 488 형광 접합체입니다. Flow Cytometry 분석용으로 검증되었으며, 499/520 nm의 여기/방출 특성을 가집니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), Alexa Fluor 488, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RM134L |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2784754 |
Additional Formats
Product Specific Information
The RM134L monoclonal antibody reacts with mouse CD252 (OX-40 Ligand), a TNF superfamily member. OX-40L is induced on mouse splenic B cells stimulated with anti-IgM and anti-CD40, but not expressed on resting or activated mouse T cells.
OX-40 (CD134) interaction with OX-40L is involved in T cell-dependent humoral response. RM134L inhibits costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal dilution should be empirically determined.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
TNFSF4 (OX40 Ligand) is a type II membrane-bound, non-covalently linked homotrimeric protein expressed on antigen-presenting cells (dendritic cells, activated B cells), vascular endothelial cells, mast cells, and NK cells.
It signals through TNFRSF4 (OX40 receptor), mainly on CD4+ T cells and activated CD8+ T cells, functioning as a costimulatory signal enhancing proliferation, survival, and cytokine expression (RANTES, IL-2, IL-3, IFNγ).
TNFRSF4/TNFSF4 signaling is critical for immunotolerance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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