Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Biotin
• eFluor 450
• eFluor 660
• PE
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi.
There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells (BALB/c > C57BL/6).
GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, recognized by CD22.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Careful titration recommended for optimal assay performance.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

GL7 is a cell-surface protein found on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi.
Expression varies by strain (BALB/c > C57BL/6).
Commonly used as a marker for mouse germinal center B cells.
The GL7 epitope is a sialic acid glycan moiety (Neu5Ac) recognized by CD22.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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