Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor 488, eBioscience
GL7 단클론 항체로 활성화된 T/B 림프구 및 생쥐 생식중심 B세포를 표지하는 데 사용됩니다. Alexa Fluor 488 형광 표지로 흐름세포분석에 최적화되어 있으며, 인간 및 생쥐 반응성. 0.5 µg/test 농도로 사용 권장, 4°C 암소 보관.
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Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 20 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgM |
| Recommended Isotype Control | Rat IgM Isotype Control (eBRM), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL-7 (GL7) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016647 |
Additional Formats Available
- Unconjugated
- Biotin
- eFluor 450
- eFluor 660
- PE
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi.
There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells (BALB/c > C57BL/6).
GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, recognized by CD22.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Careful titration recommended for optimal assay performance.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
GL7 is a cell-surface protein found on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi.
Expression varies by strain (BALB/c > C57BL/6).
Commonly used as a marker for mouse germinal center B cells.
The GL7 epitope is a sialic acid glycan moiety (Neu5Ac) recognized by CD22.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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