Thermo Fisher Scientific c-Jun Recombinant Rabbit Monoclonal Antibody (2H4L1)
Recombinant rabbit monoclonal antibody targeting c-Jun for WB, ICC/IF, and ChIP applications. High specificity and sensitivity with consistent lot performance. Reacts with human and mouse; predicted reactivity with monkey, bovine, and pig. Supplied unc...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250–1:2,500 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg × 10^6 cells | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2H4L1 |
| Immunogen | Peptides corresponding to Human JUN (aa 21–36, 211–228) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633062 |
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
c-Jun is a transcription factor that binds the enhancer motif 5'-TGA[CG]TCA-3'. It promotes NR5A1 activity when phosphorylated by HIPK3, increasing steroidogenic gene expression upon cAMP pathway stimulation. Also involved in KRAS-mediated activation of USP28 in colorectal cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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