Thermo Fisher Scientific Phospho-Vimentin (Ser39) Recombinant Rabbit Monoclonal Antibody (3H9L2)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Cat, Pig, and Sheep.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the large rabbit immune repertoire

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs.

Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangements that facilitate cholesterol transport to mitochondria, enhancing steroid production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.