Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), NovaFluor Yellow 730, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), NovaFluor Yellow 730, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test
Publications: -

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The SK1 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimer or CD8α/α homodimer. CD8a is present on thymocytes, a subset of mature T cells, and NK cells. CD8 binds MHC class I and interacts with protein tyrosine kinase p56lck, contributing to T-cell development and activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended usage: 5 µL (0.2 µg) per test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample before combining antibodies with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 718 nm
Laser: 561 nm (Yellow)

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays a key role in T-cell signal transduction, differentiation, and activation. CD8 is expressed on T cells as a disulfide-linked heterodimer (CD8α/β) functioning as a co-receptor for MHC class I molecules, while CD4 serves for MHC-II. CD8 also exists as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.

Upon MHC-I/peptide complex binding, CD8 recruits Lck kinase, triggering lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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