Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Species Reactivity

Human

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SK1

Conjugate

NovaFluor™ Yellow 755

Additional Formats:

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Excitation / Emission Max

551 / 755 nm
Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow)

Product Specifications

Product Specific Information

The SK1 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimers or CD8α/α homodimers. CD8a is found on thymocytes, mature T cells, and NK cells. It binds MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T-cell development and activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use at 5 µL (0.06 µg) per test, defined as staining a cell sample in 100 µL volume (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  Add 5 µL per sample to antibody cocktails before combining with cells.
  For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology, enabling engineered fluorescent signatures optimized for reduced spillover and spectral spread.

Target Information

CD8 is a type I transmembrane glycoprotein functioning as a co-receptor with TCR for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8α binds MHC-I and recruits Lck kinase, leading to CTL activation and pathogen/tumor clearance. Differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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