Thermo Fisher Scientific IL-6 Monoclonal Antibody (MQ2-13A5), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• Functional Grade
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21–28 kDa cytokine secreted by various cell types.
This antibody is reported for use in intracellular flow cytometric analysis.

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells.
Recommended usage ≤ 0.25 µg per test (100 µL final volume).
Cell number per test: 10⁵–10⁸ cells (empirically determined).
Careful titration is recommended for optimal performance.

Excitation / Emission: 488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-6 encodes a cytokine involved in inflammation and B-cell maturation.
It acts as an endogenous pyrogen that can induce fever in autoimmune or infectious diseases.
IL-6 is primarily expressed at inflammation sites and secreted into serum, inducing transcriptional inflammatory responses via the IL-6 receptor alpha.
It is implicated in various inflammation-associated diseases such as diabetes mellitus, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and rheumatoid arthritis.
Elevated IL-6 levels have been observed in viral infections, including COVID-19 (SARS-CoV-2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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