
Thermo Fisher Scientific CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), PE, eBioscience
인간 MCP-1(CCL2)을 인식하는 단일클론 항체로, PE 형광물질로 결합되어 세포내 염색 및 유세포분석에 적합합니다. 5D3-F7 클론, 마우스 IgG1 아이소타입이며 0.2 mg/mL 농도의 액상 형태로 제공됩니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), PE, eBioscience™
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 0.125 µg/test
View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D3-F7 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572652 |
Additional Formats
Product Specific Information
The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey MCP-1 (CCL2), also known as MCAF. MCP-1 is a CC chemokine dimer derived from monocytes and vascular endothelial cells, showing chemoattractant activity for monocytes, basophils, T cells, and NK cells via CCR2, CCR5, and CCR10.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) according to BestProtocols®: Protocol A.
Recommended usage ≤ 0.125 µg per test (100 µL final volume). Cell numbers may vary (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Spectral Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CCL2 (MCP-1) is a cytokine gene located on chromosome 17q, part of the cytokine family involved in immunoregulatory and inflammatory processes. It shows chemotactic activity for monocytes and basophils, but not for neutrophils or eosinophils, and is implicated in diseases such as psoriasis, rheumatoid arthritis, and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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