Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), PE, eBioscience

Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 78 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine, and functions as a neutralizing antibody. Mouse IL-4 (also known as BCDF, BCGF-1, BSF-1) is secreted primarily by activated Th2 and natural killer T cells, and promotes B cell proliferation and differentiation. It induces Ig isotype switching and upregulation of surface MHC class II and CD23.

Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Validated for intracellular staining of Th2-polarized and stimulated mouse splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Optimize antibody titration for best performance.

Excitation / Emission:
488–561 nm / 578 nm (Blue, Green, Yellow-Green Laser compatible)

Filtration:
0.2 µm post-manufacturing filtered

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. It also interacts with IL-13, contributing to overlapping functions. STAT6 mediates IL-4 signaling. The IL-4, IL-3, IL-5, IL-13, and CSF2 genes form a cytokine cluster on chromosome 5q, with IL-4 located near IL-13. IL-4, IL-13, and IL-5 are co-regulated by long-range elements over ~120 kb. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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