Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• eFluor 660
• FITC
• PE
• Request custom conjugation available

Product Specific Information

The eBioH4B4 monoclonal antibody reacts with human CD107b (LAMP-2), a highly glycosylated type I transmembrane protein (~105 kDa) localized in lysosomal/endosomal membranes. It is also expressed on the surface of degranulating T cells, activated platelets, and certain cancer cells. Mutations in CD107b cause Danon disease, a lysosomal glycogen storage disorder.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Surface staining in flow-based degranulation assay

Applications Tested:

• Pre-titrated and validated for intracellular staining and flow cytometry using Jurkat cells
• Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Excitation: 488 nm
Emission: 519 nm
Laser: Blue laser

Target Information

LAMP2 (CD107b) is a lysosome-associated membrane glycoprotein involved in lysosomal biogenesis, fusion with phagosomes, and chaperone-mediated autophagy. It is a ~46 kDa precursor that becomes 100–130 kDa after glycosylation. LAMP2 has three isoforms (LAMP-2A, -2B, -2C) and functions with LAMP1 in lysosomal membrane integrity. Deficiency leads to autophagosome accumulation and Danon disease (cardiomyopathy, myopathy).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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