Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), Alexa Fluor 488, eBioscience
인간 CD107b(LAMP-2)를 인식하는 Alexa Fluor 488 결합 단일클론 항체로, 세포 내 염색 및 유세포분석에 적합. 높은 특이성과 재현성을 제공하며, Danon 병 관련 연구 및 라이소좀 단백질 분석에 활용 가능. 연구용 전용 제품.
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Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 0.25 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4B4 (H4B4) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596504 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- Unconjugated
- eFluor 450
- eFluor 660
- FITC
- PE
- Request custom conjugation available
Product Specific Information
The eBioH4B4 monoclonal antibody reacts with human CD107b (LAMP-2), a highly glycosylated type I transmembrane protein (~105 kDa) localized in lysosomal/endosomal membranes. It is also expressed on the surface of degranulating T cells, activated platelets, and certain cancer cells. Mutations in CD107b cause Danon disease, a lysosomal glycogen storage disorder.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow-based degranulation assay
Applications Tested:
- Pre-titrated and validated for intracellular staining and flow cytometry using Jurkat cells
- Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Excitation: 488 nm
Emission: 519 nm
Laser: Blue laser
Target Information
LAMP2 (CD107b) is a lysosome-associated membrane glycoprotein involved in lysosomal biogenesis, fusion with phagosomes, and chaperone-mediated autophagy. It is a ~46 kDa precursor that becomes 100–130 kDa after glycosylation. LAMP2 has three isoforms (LAMP-2A, -2B, -2C) and functions with LAMP1 in lysosomal membrane integrity. Deficiency leads to autophagosome accumulation and Danon disease (cardiomyopathy, myopathy).
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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