Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor 488, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 2 µg/test
• Publications: View 13 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• PE
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40–50 kDa member of the TNFR superfamily.
CD95 is expressed by a wide range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes, and fibroblasts.
Interaction of CD95 with its ligand (FasL) induces apoptosis and plays a role in peripheral tolerance.
15A7 reacts strongly with mouse CD95 in BALB/c strain and more weakly in C57Bl/6 strain. It does not induce apoptosis of Fas-expressing cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymocytes, ≤2 µg/test (100 µL final volume).
Recommended Laser: Blue laser (Excitation 488 nm; Emission 519 nm)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor of the TNFR superfamily.
It also exists in a soluble 26 kDa form. Stimulation of FAS leads to aggregation of FADD domains, formation of the death-inducing signaling complex (DISC), and activation of caspases, resulting in apoptosis.
FAS can also activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and mediates proliferative signals in fibroblasts and T cells.
Multiple alternatively spliced variants exist, with some lacking the transmembrane domain and acting as negative regulators of apoptosis.
The Fas/FasL pathway is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a role in antiviral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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