Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Alexa Fluor 488, eBioscience
Alexa Fluor 488으로 표지된 eBio1D4B 클론 CD107a(LAMP-1) 단클론 항체로, 마우스 시료에 반응합니다. Flow Cytometry, ICC/IF, Western Blot에 적합하며, 세포 탈과립 및 리소좀 관련 마커 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제 및 4°C 암소 보관.
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Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Alexa Fluor 488
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 8 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 12 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D4B (1D4B) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657537 |
Available Formats
- Unconjugated
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I lysosomal membrane protein that is extensively glycosylated and expressed constitutively in late endosomes and lysosomes. It is transiently expressed on the surface of degranulating cytolytic T cells and is implicated in cancer metastasis and lysosomal storage disorders.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric degranulation assays
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. Recommended use ≤ 0.5 µg/test (100 µL final volume). Optimal titration advised.
Additional Details:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a) is a major lysosomal membrane component, together with LAMP2. Approximately 1–2% of total CD107a is found on the plasma membrane. LAMP1 is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes. Its surface expression is associated with cell activation and adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed around senile plaques in Alzheimer’s disease and in activated mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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