Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), APC, eBioscience
CD47 단백질을 인식하는 Rat IgG2a 단클론 항체로, APC 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며 마우스 시료에 반응합니다. 0.2 mg/mL 농도의 액상 형태로, 4°C 암소 보관이 필요합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 17-0471-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), APC, eBioscience
Applications
- Flow Cytometry (Flow)
Tested dilution: 1 µg/test
View 6 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | miap301 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1724085 |
Additional Formats
Product Specific Information
The monoclonal antibody miap301 reacts to CD47 (also known as integrin-associated protein, IAP, and neurophilin).
CD47 is a glycosylated five-transmembrane protein with a small cytoplasmic domain. It is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins (CD51/CD61, CD41/CD61). It also serves as a receptor for thrombospondin.
CD47 expression is found in most hematopoietic cells (T/B cells, monocytes, platelets, erythrocytes) as well as non-hematopoietic cells. No cross-reactivity is seen against human CD47.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes
- Recommended ≤ 1 µg/test
- Defined as amount staining a 100 µL cell sample (10⁵–10⁸ cells/test)
- Optimize titration for best performance
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates their functions. Engagement of CD47 modulates various signaling pathways, including activation of heterotrimeric G proteins and intracellular calcium increase upon adhesion to the extracellular matrix.
CD47 also acts as a receptor for the C-terminal domain of thrombospondin and plays roles in membrane transport and signal transduction. It binds CD172a (SIRPα) on macrophages, preventing phagocytosis of CD47-positive cells. Additionally, the CD47–CD172a interaction influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, with high expression in synapse-rich brain and retina regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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