Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), APC, eBioscience
Flow cytometry용 APC 형광 표지 CD45R(B220) 단클론 항체로, Mouse 및 Human 반응성. B 세포 계통 및 일부 T/NK 세포 표지에 적합. 0.25 µg/test 농도로 최적화된 분석 가능. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 17-0452-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), APC, eBioscience
Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), APC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 185 publications |
| ELISA (ELISA) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469394 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450–660 series
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 series
- Custom conjugation available
Product Specific Information
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by B cell lineage from early Pro-B to mature B cells. Some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in bone marrow, and T cells of lpr/lpr mutant mice also express this antigen.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse spleen cells; optimal performance at ≤0.25 µg/test.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, regulating cellular processes such as growth, differentiation, and signal transduction. CD45 is specifically expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling. It exists in multiple isoforms via alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. CD45 plays roles in lymphocyte development, integrin-mediated signaling, and apoptosis. CD45RA is a restricted isoform among lymphoid subtypes. Dysfunction is associated with Severe Combined Immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.