Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), APC, eBioscience
인간 CD45를 인식하는 HI30 단일클론 항체로, APC 형광체가 결합된 유세포분석용 항체입니다. 모든 백혈구에서 발현되는 CD45 검출에 적합하며, 5 µL/test로 사용 가능합니다. 4°C 보관, 동결 금지. 연구용으로만 사용됩니다.
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- 17-0459-4x (2개 옵션)
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 59 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI30 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10671389 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 585, 690
- NovaFluor Red 710, 725, 755
- NovaFluor Yellow 590, 660, 730, 755
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
Description:
The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic region of CD45 has tyrosine phosphatase activity, playing a key role in lymphocyte activation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Excitation/Emission:
633–647 nm / 660 nm (Red Laser)
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines.
CD45 plays a critical role in lymphocyte development and antigen signaling, regulating Src-family kinases and cytokine receptor signaling. It exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45 isoforms (e.g., CD45RA) show cell-type–specific expression and influence signal transduction, apoptosis, and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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