Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 585, 690
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 590, 660, 730, 755
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

Description:
The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic region of CD45 has tyrosine phosphatase activity, playing a key role in lymphocyte activation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Excitation/Emission:
633–647 nm / 660 nm (Red Laser)

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines.
CD45 plays a critical role in lymphocyte development and antigen signaling, regulating Src-family kinases and cytokine receptor signaling. It exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45 isoforms (e.g., CD45RA) show cell-type–specific expression and influence signal transduction, apoptosis, and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.