Thermo Fisher Scientific AIF Recombinant Rabbit Monoclonal Antibody (SZ05-01)
AIF 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 동물 유래 성분이 없는 일관된 로트 품질 보장. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ05-01 |
| Immunogen | Synthetic peptide within Human AIF aa 502–551 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809326 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates. Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF localizes in mitochondria and translocates to the nucleus upon apoptosis induction, releasing apoptogenic proteins such as cytochrome c and caspase-9. It is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Alternative splicing produces multiple transcript variants, and a pseudogene exists on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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