Thermo Fisher Scientific Bax Recombinant Rabbit Monoclonal Antibody (SZ3-07)
BAX 단백질을 인식하는 재조합 토끼 모노클로날 항체로, Western blot과 유세포분석에 적합합니다. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분이 없는 포뮬레이션을 제공합니다. 세포 사멸 연구 및 Bcl-2 패밀리 단백질 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:20,000
- View 5 publications
Flow Cytometry (Flow)
- Tested Dilution: 1:1,000
- View publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ3-07 |
| Immunogen | Synthetic peptide within Human Bax aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809325 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
BAX is a member of the Bcl-2 family and plays a crucial role in apoptosis regulation.
- Bcl-2: anti-apoptotic
- BAX: pro-apoptotic
Apoptosis regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms.
- Bax α (21 kDa) and Bax β (24 kDa) contain BH1, BH2, BH3 domains
- Bax β lacks a hydrophobic transmembrane domain
- Bcl-2 α (26 kDa) and Bcl-2 β (22 kDa) differ in UTR and coding regions
Overexpression of BAX promotes cell death through dimerization with BCL-2 related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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