Thermo Fisher Scientific PGR Monoclonal Antibody (OTI34G6), TrueMAB

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

• Dilution: 1:150

Immunocytochemistry (ICC/IF)

• Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. It mediates the physiological effects of progesterone, which plays a central role in reproductive processes such as pregnancy establishment and maintenance.
PR exists as two isoforms: PR-A (94 kDa) and PR-B (120 kDa). PR-B is transcriptionally active, regulating genes for endometrial maintenance and ovulation inhibition. PR-A differs by a 165 amino acid deletion at the N-terminus, exposing an inhibitory domain that represses steroid hormone transcriptional activity.
In its inactive state, PgR forms a multiprotein complex with heat shock proteins and immunophilins. Upon progesterone binding, the receptor undergoes conformational changes, dimerizes, and binds to progesterone response elements (PRE), activating transcription.
A null mutation in the PGR gene leads to reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.