Thermo Fisher Scientific CD262 (DR5) Monoclonal Antibody (MD5-1), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

Description:
The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.

Applications Reported:
The MD5-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. It can be used at ≤0.125 µg per test (defined as the amount of antibody for a 100 µL staining volume). Cell numbers may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a death domain-containing receptor for TRAIL, mediating TRAIL-induced apoptosis. It belongs to the TNF-receptor superfamily and contains an intracellular death domain. DR5 activation by TNFSF10/TRAIL/APO-2L transduces apoptosis signals. Studies with FADD-deficient mice show that FADD is required for DR5-mediated apoptosis. Two transcript variants encoding different isoforms and one non-coding transcript have been identified. Overexpression of DR5 induces apoptosis and activates NF-kappaB. DR5 is expressed in various cell types, especially lymphocytes and spleen. Dysregulation is associated with squamous cell carcinoma and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.